Home Preprocessing Baseline Correction  
See also: Baseline Format Specs, CorrectBaseLine


Baseline Correction 

Epina ImageLab offers several ways to correct the baseline of spectra: the user may select either from polynomial fits through pivot points, from a penalized spline through pivot points, from the Lieber algorithm [Lieber 2003] or from an asymmetric least squares approach (Eilers algorithm,[Eilers 2005]). The basic idea behind the baseline correction (using polynomials and splines) is the construction of a baseline by interpolating up to 30 pivot points along the xaxis of the spectrum. The pivot points can be edited interactively (add, remove, adjust position) by clicking and/or dragging the pivot points directly in the spectrum. The yvalue of each pivot point is determined by the mean of k neighboring values. The number of neighbors can be specified by the user ("Neighborhood" control). Further, the order of the polynomial and the "stiffness" of the spline can also be adjusted. Please note that the stiffness parameter allows you to vary the splinebased interpolation between a normal spline (stiffness = 0.0) and a linear regression line (stiffness = 1.0).
The algorithm of Lieber is an iterative one. It uses a polynomial interpolation of all data points, cutting all data points above the current interpolation curve and setting them to the corresponding points of the polynomial. After n iterations the algorithm converges forming a polynomial baseline of order d. Both the number of iterations n and the order of the polynomial d can be set by the user.
In many cases the best way to correct the baseline is the asymmetric least squares approach developed my Eilers et al. However the Eilers algorithm is the slowest of all options, for large images it would be a good idea to run the correction overnight. The basic idea of this approach is to weight low intensities more strongly than high intensities ("Asymmetry" control). Together with a regularisation parameter ("Smoothness") this algorithm generates a smooth baseline which adapts itself to the given data. Typical values for the two parameters are 0.001 for Asymmetry and 100000 for Smoothness.
In order to perform a baseline correction of a multisensor dataset, you have to select the spectra which the correction should be applied to. Further Epina ImageLab allows you to restrict the baseline correction to a certain range of the spectrum. The range to be processed can be adjusted for each spectral group of the multisensor dataset separately.
